Complex study of physical and chemical properties of biological objects

Journal: Volume 21, No. 1, 2016

Pages: 67 – 71

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Complex study of physical and chemical properties of biological objects

Abstract

The aim of this work is to study and analyze various types of human hair (both processed by various chemicals and non-processed) by atomic force microscopy (AFM) to determine the degree and causes of damage depending on the type of chemical treatment. In this paper, the author has researched human hair of various types with preliminary diverse chemical treatment in order to compare and identify the mechanism of influence of various things on the hair-covering state. The paper describes a research method of biological objects (for example, of human hair) using AFM in contact mode. The possibility of safe electrostatic bindings of objects on surface-supported has been shown and the optimal modes of research have been selected. It is shown that cone-shaped probe with rake angle of about 70°, the edge radius of about 50 nm, fixed on the console with hardness not exceeding 0.05 N/m to minimize artifacts, such as contact deformation and convolution. The results of the research of four hair types, which has been undergone by kinds of chemical treatment, have been analyzed and it is found that the use of chemicals for hair treatment strongly affects its geometrical parameters. It is shown that the use of chemicals for coloring and perming leads to hair stratification (maximum of roughness difference is 450 nm) and in the result it is possible to lose the hair’s ability to retain moisture and cause a breach of homogeneous conductivity of nutrients. Through studies of uncolored, natural hair there was no contrast of relief distribution and it indicates normal distribution of nutrients on the hair shaft. AFM images of gray hair indicate scales increase, however microgeometry of hair shaft is well-developed (compared to microgeometry of colored hair with perming)

Keywords

atomic force microscopy; biological objects; preparation of biological samples; chemical hair treatment

References

Antonyuk, V.S., Bilokin, S.O., Bondarenko, M.O., et al. (2015). Formation of wear-resistant coatings on silicon probes for atomic force microscopy by thermal vacuum evaporation. Superhard Materials, (2), 60–69.
Antonyuk, V.S., Bondarenko, Yu.Yu., Bondarenko, M.O., et al. (2014). Prospects for using atomic force microscopy for integrated control of precision instrument elements. Advanced Technologies and Devices: Collection of Scientific Papers, (5), 5–9.
Antonyuk, V.S., et al. (2013). Microscopy in nanotechnologies: Monograph. Kyiv: NTUU "KPI".
Antonyuk, V.S., et al. (2014). Methods and tools of microscopy: Monograph. Kyiv: NTUU "KPI".
Antonyuk, V.S., Khrapatyi, S.V., Bilokin, S.O., & Andriyenko, V.O. (2014). Formation of functional nanostructures on dielectric surfaces by thermal vacuum evaporation. Bulletin of the National Technical University of Ukraine “Kyiv Polytechnic Institute”, (1)70, 31–35. (Series: Mechanical Engineering).
Bilokin, S.O., Antonyuk, V.S., Andriyenko, V.O., & Khrapatyi, S.V. (2014). Modeling of electrostatic interaction of AFM probe with dielectric surface. Collected Scientific Works, (2)41, 233–237. (Series: Industrial Engineering, Construction).
Bilokon, S.A., Bondarenko, M.A., Antonyuk, V.S., & Andriyenko, V.A. (2013). Study of microhardness of thin films using nanoindentation by atomic force microscopy. In Quality, Standardization, Control: Theory and Practice: Proc. of the 13th Int. Sci.-Pract. Conf., Sept. 30 – Oct. 4, Crimea, Yalta, Kyiv (pp. 20–21).
Bilokon, S.A., Bondarenko, M.A., Bondarenko, Yu.Yu., et al. (2014). Minimization of capillary force influence when studying surfaces of nanoelectronics products in contact mode of AFM. In Methodological aspects of scanning probe microscopy: Proc. of the 11th Int. Conf., Oct. 21–24, Minsk, Belarus (pp. 164–168).
Dedkova, E.G., et al. (2011). Instruments and methods of probe microscopy. Moscow: Mozhaisky Printing Plant.
Drozd, E.S., & Chizhik, S.A. (2010). A method for evaluating local elastic properties of biological cells based on atomic force microscopy. In Methodological aspects of scanning probe microscopy: Proc. of the 9th Int. Conf., October 12–15, Minsk (pp. 124–129).

Suggested citation

(2016). Complex study of physical and chemical properties of biological objects. Bulletin of Cherkasy State Technological University, 21(1), 67-71.

[1] Antonyuk, V.S., Bilokin, S.O., Bondarenko, M.O., et al. (2015). Formation of wear-resistant coatings on silicon probes for atomic force microscopy by thermal vacuum evaporation. Superhard Materials, (2), 60–69.

[2] Antonyuk, V.S., Bondarenko, Yu.Yu., Bondarenko, M.O., et al. (2014). Prospects for using atomic force microscopy for integrated control of precision instrument elements. Advanced Technologies and Devices: Collection of Scientific Papers, (5), 5–9.

[3] Antonyuk, V.S., et al. (2013). Microscopy in nanotechnologies: Monograph. Kyiv: NTUU "KPI".

[4] Antonyuk, V.S., et al. (2014). Methods and tools of microscopy: Monograph. Kyiv: NTUU "KPI".

[5] Antonyuk, V.S., Khrapatyi, S.V., Bilokin, S.O., & Andriyenko, V.O. (2014). Formation of functional nanostructures on dielectric surfaces by thermal vacuum evaporation. Bulletin of the National Technical University of Ukraine “Kyiv Polytechnic Institute”, (1)70, 31–35. (Series: Mechanical Engineering).

[6] Bilokin, S.O., Antonyuk, V.S., Andriyenko, V.O., & Khrapatyi, S.V. (2014). Modeling of electrostatic interaction of AFM probe with dielectric surface. Collected Scientific Works, (2)41, 233–237. (Series: Industrial Engineering, Construction).

[7] Bilokon, S.A., Bondarenko, M.A., Antonyuk, V.S., & Andriyenko, V.A. (2013). Study of microhardness of thin films using nanoindentation by atomic force microscopy. In Quality, Standardization, Control: Theory and Practice: Proc. of the 13th Int. Sci.-Pract. Conf., Sept. 30 – Oct. 4, Crimea, Yalta, Kyiv (pp. 20–21).

[8] Bilokon, S.A., Bondarenko, M.A., Bondarenko, Yu.Yu., et al. (2014). Minimization of capillary force influence when studying surfaces of nanoelectronics products in contact mode of AFM. In Methodological aspects of scanning probe microscopy: Proc. of the 11th Int. Conf., Oct. 21–24, Minsk, Belarus (pp. 164–168).

[9] Dedkova, E.G., et al. (2011). Instruments and methods of probe microscopy. Moscow: Mozhaisky Printing Plant.

[10] Drozd, E.S., & Chizhik, S.A. (2010). A method for evaluating local elastic properties of biological cells based on atomic force microscopy. In Methodological aspects of scanning probe microscopy: Proc. of the 9th Int. Conf., October 12–15, Minsk (pp. 124–129).